The optimization of the Genomic-SSR reaction system is a basic protocol when the Genomic-SSR is used for genetic analysis in Liriodendron. The concentrations of Mg2+, dNTP, Primer and rTaq were tested by L9(34) orthogonal experiment and single factor gradient experiment to gain the optimal reaction system. The results indicated that the optimal reaction system should contain 75 ng of genomic DNA, 1 μL of 10×buffer, 0.4 μL of 10 mmol·L-1 dNTP, 0.75 μL of 2.5 mmol·L-1 MgCl2, 0.25μL of 10 μmol·L-1 Primer, 0.05 μL of 5 U rTaq and final volume of 10μL. Repeated trials and two verification tests showed that this optimal reaction system was stable, reliable, efficient and suitable for the applications of Genomic-SSR in Liriodendron population genetics and quantitative genetics research.

以杉木成年优良无性系的无菌组培苗为外植体,研究激素、活性炭等对不定芽增殖的影响,在此基础上探讨接种方式对杉木增殖效率的影响。研究表明:6-BA浓度为1.5 mg·L-1时能有效促进不定芽增殖,但继续添加则起抑制作用,并容易引起玻璃化;去除顶端优势的接种方式能将不定芽的增殖效率提高一倍多;不同的基因型间平均不定芽数相差1;BA、KT、NAA的组合使用较单一激素使用有利于不定芽的伸长,适量的活性炭有利于杉木不定芽的伸长生长。

为了解紫外线辐射时间对杉木生长的影响,本研究以杉木优良杂交组合的体胚再生植株为材料,用紫外灯模拟自然界UV-B辐射对其进行处理,研究0、1、3、5、7、9天紫外胁迫下杉木的光合作用效率及生理状态的变化。结果表明:随着紫外线辐射时间的延长,杉木体胚再生植株叶片的叶绿素含量明显降低、叶绿素荧光参数(Fv/Fm、YⅡ、qP、qN)数值均降低,MDA含量先降低后增加,POD活性提高、可溶性蛋白含量明显增加;紫外线辐射3天叶片杉木体胚再生植株叶片尖端开始变黄, 5-7天黄化现象加剧,9天叶片完全失水,干枯死亡,说明长时间紫外辐射会阻碍杉木体胚再生植株的生长发育。